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Related Articles from SNS
LC-MS Metabolomics Reveals No Significant Impact of Microbial Inoculation with Bacillus velezensis and Lachnum sp. on Cranberry Metabolome
Sustainable agriculture has driven increased exploration of microbial inoculants as a promising strategy to boost plant growth for higher yield and enhance secondary metabolism to increase crop nutritional value. However, their influence on fruit-specific metabolites under field conditions remains understudied. This study investigated the impact of inoculating cranberry (Vaccinium macrocarpon) plants with Bacillus velezensis EB37 and Lachnum sp. EC5, applied individually and in combination,...
Discovery of BilV reveals a multienzymatic basis for bilirubin reduction across vertebrate gut microbiomes
Gut bacteria reduce bilirubin to urobilinogen, allowing it to be excreted through feces and urine, but studies have long noted a heterogeneous mixture of partially reduced bilirubin-derived intermediates, suggesting that multiple enzymes are involved. Here we identify bilirubin vinyl reductase (BilV), a novel Old Yellow Enzyme family reductase encoded in the genomic neighborhood of the known bilirubin reductase (bilR). Using heterologous expression and LC-MS/MS, we show that BilR acts on the...
RISOP, a Reference-Assisted Approach for Enhanced Identification of Oxidized Phospholipids
Oxidized phospholipids (OxPLs) play critical roles in inflammation, ferroptosis, and other oxidative stress-associated processes, yet their systematic characterization in biological systems remains a major analytical challenge owing to their low abundance and vast structural diversity. Here we report RISOP (Reference-Assisted Identification of Sample-specific Oxidized Phospholipids), an untargeted LC-MS/MS workflow that leverages chemically enriched OxPL reference pools to substantially...
A new capability to detect chemical weapons involves two existing methods
A new capability to detect chemical weapons involves two existing methods Stephanie Baum Scientific Editor Robert Egan Associate Editor In the aftermath of suspected chemical attacks, investigators from the Organization for the Prohibition of Chemical Weapons (OPCW) step in to collect chemical, environmental, and biomedical samples. Thorough forensic laboratory analysis of these samples is essential for proving what—if any—chemical agents were used and verifying their identities. Researchers...
HNSW-MS: Hierarchical Graph Indexing Enables Accurate Real-Time Mass Spectral Similarity Search at Repository Scale
Spectral similarity search is the basis of mass spectrometry-based metabolomics, underpinning library matching, molecular networks construction, and repository searches such as MASST. Until recently, dataset sizes were limited, making exhaustive pairwise comparison tractable. This is no longer true.
From definition to discovery: metabolite markers of high temperature in green grape berries
Understanding how plants respond to high temperature is critical under global warming. Metabolite markers can provide insights into stress-responsive mechanisms and help guide strategies to maintain crop quality. However, heat-associated metabolite markers in grape berries remain poorly defined, particularly at the green stage, a critical phase of berry development during which early metabolic perturbations can influence subsequent ripening and ultimately determine berry composition and quality.
Bacteroides fragilis modulates gut microbiome community composition, frontal cortex gene expression, and fecal and frontal cortex metabolites in a mouse model of Alzheimers disease pathologies
Alzheimers disease (AD) is a neurodegenerative disease and the leading cause of dementia among elderly. Gut microbiome alterations precede pathogenesis and may affect disease outcomes. We evaluated the role of Bacteroides fragilis in triple transgenic mice modeling AD pathologies (3xTg-AD) and wild-type controls (WT).
Transcriptomic and proteomic responses to gas vesicle collapse in native and engineered bacterial systems
Gas vesicles (GVs) are air-filled protein nanostructures produced by microbes to regulate buoyancy and have emerged as powerful tools in biomedical imaging, particularly as acoustic reporter genes. Their mechanically robust shells enclose a stable air compartment, which collapses irreversibly when subjected to sufficient hydrostatic or acoustic pressure, leaving behind large protein sheets. This collapse phenomenon underlies key applications such as differential imaging and controlled...
The Sorghum Lipid Database (SoLD): population-scale lipidomics linking environmental and genetic variation in the Sorghum Association Panel
Sorghum (Sorghum bicolor ) is a climate-resilient crop whose acclimation to nutrient limitation and low temperature likely involves extensive lipidome reconfiguration. Lipids are key membrane components, carbon and energy stores, and mediators of stress signaling, yet population-scale lipidomics data for sorghum are limited. We present the Sorghum Lipid Database (SoLD), a curated lipidomics resource from the Sorghum Association Panel grown under two field regimes: (i) a nutrient-sufficient...
Faster lower-cost PFAS testing could reshape how US drinking water is monitored
Faster lower-cost PFAS testing could reshape how US drinking water is monitored Gaby Clark Scientific Editor Robert Egan Associate Editor A new investigation from the University of Kansas improves detection of PFAS, a family of so-called "forever chemicals" in drinking water supplies. The method, which can measure trace pollution levels of PFAS in water more quickly and inexpensively than current techniques, was recently detailed in the journal PLOS Water. PFAS chemicals, marketed for...