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Optical Photothermal Infrared Images Cells

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Label-Free AI-Classification of Subcellular Organelles Based on Optical Photothermal Infrared Images

Cells maintain homeostasis by dynamically reorganizing their organelles to tune metabolism in response to stress. Fluorescence microscopy maps organelle locations with subcellular resolution but provides limited information on their chemical composition. Infrared (IR) imaging offers a label-free alternative for probing intrinsic molecular vibrations that report on lipids, carbohydrates, and nucleic acids.

bioRxiv 5d ago