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Related Articles from SNS

Phosphorylation-dependent regulation of Hsp70 chaperones stimulates client recruitment

Molecular chaperones of the Hsp70 family play essential roles in maintaining proteostasis, particularly under conditions of cellular stress. Posttranslational modifications of Hsp70, collectively termed the chaperone code, are emerging as critical regulators of chaperone function, yet their mechanistic contributions remain incompletely understood. Here, we investigate the functional significance of a conserved phosphorylation site in Hsp70, corresponding to serine 326 in yeast Ssa1 and...

bioRxiv 8d ago

TPR Domains Drive the Functional Phase Separation of HOP and its Regulation by Hsp90 and Hsp70

HOP is a cochaperone that facilitates client transfer between two major chaperones, Hsp90 and Hsp70. Emerging evidence, however, suggests that HOP plays additional roles in coordinating complex proteostasis networks. We demonstrate here that stress-dependent HOP foci are biomolecular condensates formed by liquid-liquid phase separation.

bioRxiv 4d ago

Comparative analysis of neuronal proteolytic pathways reveals neuron-specific and sub-compartmental-specific capacities with aging

Proteostasis is essential for maintaining neuronal function, and its dysregulation is a hallmark of aging and neurodegeneration. The ubiquitin-proteasome system (UPS) and macroautophagy are the two major proteolytic pathways responsible for protein degradation. However, their capacity and regulation differ between cell types and across aging.

bioRxiv 7d ago

Developmental genetic response of the zooplanktonic tunicate Oikopleura dioica to marine noise pollution.

Background: Anthropogenic noise is an emerging threat to marine ecosystems, yet its effects on marine invertebrates, particularly zooplanktonic species, remain poorly understood. Despite increasing evidence of behavioral and physiological impacts in invertebrates, the effects of noise on embryonic development and the molecular mechanisms underlying acoustic responses remain largely unexplored. Here, to address this gap, we investigated the impact of high-intensity underwater noise exposure...

bioRxiv 11d ago

Comparative Proteomics Across Tissues and Crop Agroecosystems Reveals Agricultural Stressor Responses in the Western Honey Bee

Maintaining honey bee health in crop production systems is increasingly difficult because worker bees encounter multiple chemical and biological pressures from pesticides and pathogens. How these field-realistic pressures affect molecular physiology across functionally distinct tissues remains poorly understood. Here, we tested whether tissue-resolved proteomics could separate stable tissue-specific patterns from crop-associated molecular changes.

bioRxiv 5d ago

Mitochondria directly interact with the nuclear pore complex

Abstract Mitochondria regulate cellular processes through direct and indirect interactions with other organelles. A well-studied example has been contact with the endoplasmic reticulum at mitochondrial-associated endoplasmic reticulum membranes1, which control pathways including redox and calcium homeostasis2,3. Recent studies have also reported direct mitochondria–nuclear membrane contacts in cancer cells and yeast that promote pro-survival signalling4,5.

Nature 1d ago

The Sorghum Lipid Database (SoLD): population-scale lipidomics linking environmental and genetic variation in the Sorghum Association Panel

Sorghum (Sorghum bicolor ) is a climate-resilient crop whose acclimation to nutrient limitation and low temperature likely involves extensive lipidome reconfiguration. Lipids are key membrane components, carbon and energy stores, and mediators of stress signaling, yet population-scale lipidomics data for sorghum are limited. We present the Sorghum Lipid Database (SoLD), a curated lipidomics resource from the Sorghum Association Panel grown under two field regimes: (i) a nutrient-sufficient...

bioRxiv 5d ago