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Tau aggregate replication occurs at the pre-synapse of cultured human neurons and increases with application of TNFa
Tau aggregation at synapses is a key process driving Alzheimer's disease but the mechanism(s) that cause this have not been established. We used a model system of forward-programming induced glutamatergic neurons (iNeurons) with three independent cell lines treated with TNFa. Using aggregate-specific SIMOA, STED microscopy, and SynPull to detect nanoscopic tau aggregates in bulk samples and at individual synapses, we found that TNFa-driven tau aggregation occurs preferentially at the...
Topological texture analysis of microscopy images of dynamic casein gelation and its relation to rheological properties
arXiv:2606.02048v1 Announce Type: cross Abstract: We propose a novel computational toolbox that integrates Topological Data Analysis (TDA), Differential Box Counting (DBC), Multifractal Partition (MFP), and Local Binary Patterns (LBP), applied to time-lapse super-resolution STED microscopy images of sodium caseinate gelation induced by glucono-delta-lactone (GDL) at 30 {\deg}C and 40 {\deg}C and two GDL concentrations (1.8% and 3.5% w/v). TDA tracked topological loops, closed ring-like...
Topological texture analysis of microscopy images of dynamic casein gelation and its relation to rheological properties
arXiv:2606.02048v1 Announce Type: new Abstract: We propose a novel computational toolbox that integrates Topological Data Analysis (TDA), Differential Box Counting (DBC), Multifractal Partition (MFP), and Local Binary Patterns (LBP), applied to time-lapse super-resolution STED microscopy images of sodium caseinate gelation induced by glucono-delta-lactone (GDL) at 30 {\deg}C and 40 {\deg}C and two GDL concentrations (1.8% and 3.5% w/v). TDA tracked topological loops, closed ring-like...
Mitochondria directly interact with the nuclear pore complex
Abstract Mitochondria regulate cellular processes through direct and indirect interactions with other organelles. A well-studied example has been contact with the endoplasmic reticulum at mitochondrial-associated endoplasmic reticulum membranes1, which control pathways including redox and calcium homeostasis2,3. Recent studies have also reported direct mitochondria–nuclear membrane contacts in cancer cells and yeast that promote pro-survival signalling4,5.