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Functional class 2 CCA-adding enzymes in Asgard archaea and their implications for eukaryogenesis

CCA-adding enzymes are essential for transfer RNA (tRNA) maturation and translation among all domains of life. A long standing dogma holds that archaea encode exclusively class one CCA-adding enzymes (CCA1), whereas bacteria and eukaryotes encode class two enzymes (CCA2), creating a perceived strict evolutionary separation between archaeal and eukaryotic CCA adding enzymes. Here, we show that this view is incorrect.

bioRxiv 10d ago

Multivariate integration of histological images and gene expression data: a comparative review

Integrating histological images with gene expression data offers a promising approach for linking tissue morphologies to molecular signatures and improving disease subtyping. However, such integration remains challenging due to the high dimensionality of these datasets, cross-modal heterogeneity, and limited interpretability. Multivariate methods such as Sparse Canonical Correlation Analysis (Sparse CCA), Joint Nonnegative Matrix Factorisation (Joint NMF), and Angle-based Joint and...

bioRxiv 4d ago