Cas9
No mentions found
This entity hasn't been tracked yet, or Iris is still building its knowledge base.
Related Articles from SNS
A phage-encoded small RNA that mimics chimeric guide to inhibit CRISPR-Cas9
CRISPR Cas9 relies on a dual crRNA-tracrRNA guide, yet whether RNA based anti CRISPRs exist for this system has remained unknown. Here we identify a phage encoded small non coding RNA, rAcrIIA1, that adopts an unexpected chimeric crRNA-tracrRNA architecture, faithfully recapitulating the entire single guide RNA (sgRNA) of Cas9. Cryo EM structures of the Cas9-rAcrIIA1 binary complex and the Cas9-rAcrIIA1-DNA ternary complex reveal that rAcrIIA1 engages Cas9 nearly identically to the native...
dCas allele sequestration (das-CRISPR): A Versatile New Method to Achieve Monoallelic Gene Editing in Mouse Embryos and in cell culture.
CRISPR-Cas9 technology is a powerful tool extensively used for genome editing in mouse and many other species. Streptococcus pyogenes Cas9 efficiently cuts both alleles in mouse zygotes leaving many edited embryos without a functional protein that might be needed to sustain development, to survive postnatally or to reproduce, thus complicating its overwhelmingly advantageous use in making gene modifications. About 25% of mouse genes are essential for embryonic development and another 7% are...
New 'SMArT' platform makes gene editing in hematopoietic stem cells more efficient and safer
New 'SMArT' platform makes gene editing in hematopoietic stem cells more efficient and safer Sadie Harley Scientific Editor Robert Egan Associate Editor A team of researchers led by Luigi Naldini at the San Raffaele Telethon Institute for Gene Therapy (SR-Tiget) has developed a new strategy to significantly improve the precision and safety of CRISPR-Cas9 gene editing in human blood stem cells, potentially overcoming one of the major barriers limiting broader clinical application of genome...
Efficient base editing and development in human embryos without chromosomal alterations
Cas9-based tools enable the introduction of genetic lesions to investigate DNA repair outcomes and edit the genome at disease-relevant loci. DNA double-strand breaks (DSBs) induced by CRISPR/Cas9 result in frequent aneuploidy and large deletions, revealing a repair deficiency in early human embryos and limiting the clinical application of this technology. Here we evaluated the DNA repair outcomes of DNA nicks and mismatches introduced using base editors in human embryos at two targets, PCSK9...
Are we getting to the point where it's safe to gene-edit babies?
When a rogue researcher in China revealed in 2018 that he had used CRISPR to create three gene-edited children, his actions were almost universally condemned by biologists around the world. The main objection was not that gene-editing babies is wrong in itself, but that the CRISPR technique used was not safe and had a very high risk of causing harmful mutations. Now, a team in the US has used an improved form of CRISPR, known as base editing, to edit healthy embryos and shown that it can be...
'All-in-one' platform developed for multiple trait stacking in crops
'All-in-one' platform developed for multiple trait stacking in crops Gaby Clark Scientific Editor Alexander Pol Deputy Editor A major goal of modern crop breeding is to efficiently combine multiple desirable traits by "stacking" the favorable gene variants (alleles) that contribute to those traits in a single crop variety. However, current strategies are often time-consuming and inefficient. Now, a team led by Gao Caixia at the Institute of Genetics and Developmental Biology of the Chinese...
An improved CRISPR-base editor tool to target virulence factors in the ruminant pathogen Mycoplasma bovis
Mycoplasma bovis is a minimal bacterium infecting cattle, which causes a wide variety of symptoms and is impacting dairy and beef producers worldwide. Part of the difficulty in research surrounding M. bovis, and other mycoplasmas, is the lack of efficient genome editing tools. We previously presented a proof of concept of a transposon-based CRISPR-Base Editor system to introduce targeted mutations in M. bovis.
Why does the Y chromosome retain UTY?
Why does the Y chromosome retain UTY? Sadie Harley Scientific Editor Andrew Zinin Lead Editor A study, published in the journal Development, is the first to precisely map endogenous UTY occupancy across the human genome and demonstrate that UTY remains functionally involved in transcriptional regulation during early human development.