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Nanopore Direct RNA Sequencing Enables Reproducible, Site-Resolved Pseudouridine Quantification in Human Ribosomal RNA
Pseudouridine is the most abundant post-transcriptional modification in human ribosomal RNA, with over 110 annotated sites and variable stoichiometry across biological contexts. Existing quantification methods are low-throughput or constrained to predefined panels. We benchmarked nanopore direct RNA sequencing using the Dorado v5.1 model against mass spectrometry-validated sites in human liver tissue, induced pluripotent stem cells, and HeLa cells.
Companies that sold gender-reveal fireworks which ignited California wildfire agree to $4m settlement
El Dorado blaze that burned 22,744 acres and claimed the life of a firefighter was ignited by an illegal deviceSign up for the Breaking News US newsletter emailNearly six years after a couple’s gender-reveal stunt sparked a deadly wildfire in southern California, the companies that sold the pyrotechnic device have agreed to a multimillion-dollar settlement. The Hubbard, Ohio-based Wholesale Fireworks Corp and its subsidiary American Fireworks Wholesale LLC have agreed to pay more than $4m,...
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