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A genetic tool targeting brain GPR75
G-protein-coupled receptor 75 (GPR75) has emerged as an important mediator in diet-induced obesity (DIO) and a promising therapeutic target for anti-obesity drugs. However, the anatomical location of GPR75 in the brain remains unclear, hindering the understanding of GPR75 biology in DIO. Here, we generated a new GPR75-GFP-Ires-Cre knockin mouse strain, in which the Cre expression is driven by the endogenous GPR75 promoter and the GFP is fused with the C-terminal of the GPR75 protein.
Generative Frontier Planning for Adaptive Peer-Referral Recruitment under Covariate-Dependent Arrivals
arXiv:2606.08360v1 Announce Type: new Abstract: Peer-referral recruitment systems such as respondent-driven sampling are critical for studying and intervening on hidden populations affected by infectious diseases. To accelerate recruitment, public health agencies must adaptively allocate limited referral resources across multiple rounds, where current decisions shape both the number and the covariates of future recruits. Prior work makes this problem tractable by assuming that referrals are...
Arabidopsis hydathodes contain a dense and heterogeneous epithem for apoplastic fluid release
Hydathodes are specialized leaf tissues that mediate guttation, the release of liquid water droplets from vascular plants. They consist of xylem endings, water pores, and the epithem. The epithem comprises small cells whose cell types and functional properties remain poorly understood.
Frozen rat chromosome springs back to life inside a mouse embryo
June 8, 2026 report Frozen rat chromosome springs back to life inside a mouse embryo Paul Arnold Author Gaby Clark Scientific Editor Robert Egan Associate Editor Scientists in Japan have developed a rat-mouse hybrid embryo from a single frozen rat chromosome transplanted into a mouse egg cell. The achievement is proof that genetic material can sometimes remain functional after cryopreservation and be expressed inside the cells of a completely different species. This is giving renewed hope to...
Developing engineering strategies to enhance the genetic stability of fatty alcohol-producing strains for production scale-up
Robust strain stability is essential for industrial-scale fatty alcohol production, as metabolic burden and toxicity not only constrain productivity but also create selective pressure for low-producing or non-producing subpopulations. This genetic instability leads to genetic heterogeneity and compromises strain performance during large-scale fermentation. In this study, we investigated the production stability of fatty alcohol-producing Yarrowia lipolytica strains and developed systematic...
Thousandfold Expansion Microscopy
Biological macromolecules, such as proteins, are made of concatenated building blocks. We hypothesized that individual protein residues could be imaged by anchoring their side chains to a swellable polymer, cleaving backbone amide bonds, and expanding residues away from each other to a degree that enables them to be visualized separately. We introduce thousandfold expansion microscopy (1000ExM), a four-network interpenetrating hydrogel architecture that enables successive expansion from...