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inGSEA: An Improved Method for Gene Set Enrichment Analysis Using a Weighted Integral Statistic

Gene Set Enrichment Analysis (GSEA) is one of the most popular methods for transcriptomic analysis, yet its statistical power is limited when the biological pathways exhibit heterogeneous or non-concordant expression patterns. We propose an improved GSEA method, textbf{in}tegral-based GSEA (inGSEA). inGSEA introduces a novel enrichment score based on the Anderson-Darling weighted integral statistic.

bioRxiv 5d ago

BRAINCELL-AID: An Agentic AI Created Brain Cell Type Resource for Community Annotation

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Molecular glue degraders of HuR suppress BRAF-mutant colorectal cancer

Abstract BRAF gain-of-function mutations, particularly BRAF(V600E), affect roughly 10% of all patients with colorectal cancer (CRC), and portend poor prognosis with limited therapeutic interventions. BRAF inhibitors such as encorafenib are ineffective due to MAPK pathway reactivation driven by BRAF dimerization. Combined inhibition of BRAF and EGFR, although approved therapies, results in short survival benefits and frequent treatment resistance and relapse1,2,3.

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