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Nanopore Direct RNA Sequencing Enables Reproducible, Site-Resolved Pseudouridine Quantification in Human Ribosomal RNA

Pseudouridine is the most abundant post-transcriptional modification in human ribosomal RNA, with over 110 annotated sites and variable stoichiometry across biological contexts. Existing quantification methods are low-throughput or constrained to predefined panels. We benchmarked nanopore direct RNA sequencing using the Dorado v5.1 model against mass spectrometry-validated sites in human liver tissue, induced pluripotent stem cells, and HeLa cells.

bioRxiv 2d ago

Beyond copy number: The regulatory architecture of mitochondrial DNA gene expression

Mitochondrial DNA (mtDNA) copy number is widely used as a biomarker for mitochondrial function and disease risk, yet its relationship to mtDNA gene expression -- one important functional output -- remains poorly understood. Prior studies examining this relationship have largely relied on heterogeneous tissue samples, where confounding by cell-type composition obscures the underlying biology. We rigorously tested this relationship in lymphoblastoid cell lines (LCLs), where we find no...

bioRxiv 11d ago

These are all the countries currently on the Foreign Office ‘do not travel’ list

These are all the countries currently on the Foreign Office ‘do not travel’ list Warnings against travel abroad from the UK have been issued for countries including Iran, Syria and Russia - Bookmark - CommentsGo to comments When planning a holiday, it’s wise to check these three things before you leave for the airport: your passport is in date, travel insurance is still valid and you’re travelling to somewhere safe according to the Foreign, Commonwealth and Development Office (FCDO)....

The Independent UK 5d ago

All the Summer Game Fest showcases you can catch today

All the Summer Game Fest showcases you can catch today You can get the latest about wholesome, story-rich and LGBTQ+ games from today's streams. The 2026 Summer Game Fest main show and Day of the Devs have already brought us launch announcements for Final Fantasy 7 Revelation, the Resident Evil: Code Veronica remake and Virtua Fighter Crossroads, as well as other news about highly anticipated games. But the event is far from over: Today, June 6, you can catch more showcases featuring games...

Engadget 4d ago