Laurentian Great Lakes

Topic modeling reveals thermally partitioned and taxonomically distinct microbial subcommunities across prokaryotes and phytoplankton in the Laurentian Great Lakes

Identifying discrete microbial assemblages and their environmental drivers across multiple biological fractions simultaneously remains a central challenge in aquatic microbial ecology. We applied an integrated analytical pipeline built around Latent Dirichlet Allocation (LDA) to an eight-year 16S rRNA amplicon time series from the Laurentian Great Lakes, spanning four size-fractionated biological blocks free-living prokaryotes, particle-associated prokaryotes, and small and large...