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Related Articles from SNS

High-avidity TCR signaling induces a distinct KLR-positive exhaustion state in human tumor-infiltrating CD8 T cells associated with immunotherapy response

Tumor-specific exhausted CD8 T cells (Tex) adopt diverse phenotypes across human cancers, but the drivers of this heterogeneity remain poorly understood. Using flow cytometry and single-cell RNA and T cell receptor (TCR) sequencing of 106,667 tumor-infiltrating CD8 T cells from head and neck squamous cell carcinoma (HNSCC) tumors, we identified and validated three Tex subsets, each with distinct clonotypes: (1) Tex-Conv, expressing conventional exhaustion genes; (2) Tex-CCR6, distinguished...

bioRxiv 8d ago

New Benchmarking Shows Limited Generalization Power of TCR Antigenic Epitope Prediction Models

arXiv:2606.04994v1 Announce Type: new Abstract: Accurate computational prediction of T cell receptor (TCR) antigen specificity would transform the study of T cell biology and enable scalable immune engineering, yet existing models lack sufficient sensitivity and specificity for broad applications. A major limitation is the absence of rigorously defined, unseen benchmark datasets that allow unbiased evaluation of model performance and generalizability. Here, we describe two complementary...

arXiv CS 6d ago

Unbiased identification of responding T cell clones from longitudinal repertoire sequencing with CloneSearch

T cells activate and expand upon interaction with cognate antigen, derived from pathogens or mutated proteins. T cell clones can be identified by their T cell receptor (TCR) which can act as a unique barcode to track their expansion. Longitudinal TCR sequencing can be used to track T cell responses to a large array of stimuli.

bioRxiv 9d ago

CaliPPer: quantifying, predicting and improving AI model performance for binding prediction

arXiv:2606.07258v1 Announce Type: new Abstract: Binding prediction models accelerate therapeutic antibody and TCR discovery, but their performance on new datasets is unpredictable, often leading to low discovery rates. Density-ratio methods (PAPE, M-CBPE) provide label-free performance estimation for binary classification, but their assumptions and aggregate-only outputs limit binding prediction on neoepitopes, antigen variants and chemical scaffolds. Here we present CaliPPer (Calibration...

arXiv CS 2d ago