Tet Trim-Away
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Tet Trim-Away: A conditional rapid protein degradation system for Tetrahymena thermophila
Tetrahymena thermophila is a ciliated protist that has played pivotal roles in biological discovery. Functional studies of Tetrahymena proteins have largely relied on gene knockouts. Because protein depletion upon knockout typically spans multiple cell cycles, compensatory mechanisms can confound phenotypic interpretation.