Xanthomonas
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Multiple approaches for CRISPR-based targeting of DNA methylation to promoters of bacterial and viral susceptibility genes in cassava
Targeted epigenetic modifications of specific gene regulatory regions have the potential to confer beneficial traits for crop improvement. Two recently developed CRISPR/Cas9-based epigenome editing tools were tested in transgenic cassava to target cytosine methylation to the promoter region of MeSWEET10a, a necessary gene for infection by the Cassava bacterial blight pathogen, Xanthomonas phaseoli pv. manihotis. The two systems leverage unique methyltransferases, and each induced distinct...
Antibiotics and copper drive compartment-specific dysbiosis and functional reprogramming in tomato microbiomes
Plant-associated microbiomes help sustain plant immunity and productivity, yet the degree to which agricultural antimicrobials reshape these microbial networks and alter disease outcomes remains poorly characterized. Here, we quantified how chemical inputs such as copper and streptomycin trigger distinct, compartment-specific dysbiosis in tomato (Solanum lycopersicum), fundamentally decoupling microbiome-mediated immunity from pathogen defense. We demonstrated that chemical disturbance...
Bacteria uncover distinct strategy to import rare sugar polymers, crystal structures show
Bacteria uncover distinct strategy to import rare sugar polymers, crystal structures show Sadie Harley Scientific Editor Andrew Zinin Lead Editor Even though sugars are often framed as simple sources of energy, they also serve as structurally complex and functionally diverse molecules that mediate interactions between organisms. Among these, β-1,2-glucans, which are a class of glucose-based polymers, stand out for their varied and sometimes subtle roles. Found across a wide range of...