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UMITIC: An unsupervised framework for the joint characterization of cellular phenotypes and spatial neighborhoods in multiplex and hyperplex immunofluorescence imaging data

Multiplexed imaging technologies enable the simultaneous measurement of dozens of protein markers while preserving context, providing a high-resolution view of tissue organization schemes. However, extracting meaningful insights from these high-dimensional datasets--particularly in hyperplex settings (>20 markers)--remains a major computational challenge, especially in the absence of annotated data. Here, we present UMITIC (Unsupervised Analysis of Multiplex Images via TIssue...

bioRxiv 9d ago