Antimycin

Coupled intracellular redox and extracellular respiration sensing for quantitative oxidative stress profiling

Abstract Quantitative assessment of cellular oxidative stress requires simultaneous measurement of intracellular redox state and extracellular respiratory activity, yet integrated sensing approaches remain limited. Here, we present a dual fluorescent sensing platform combining a genetically encoded redox biosensor (roGFP2-Tsa2{Delta}CR) with an optical oxygen sensor embedded in microwell plates for parallel, noninvasive quantification of intracellular reactive oxygen species (ROS) and oxygen...