Y. lipolytica
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Coupled intracellular redox and extracellular respiration sensing for quantitative oxidative stress profiling
Abstract Quantitative assessment of cellular oxidative stress requires simultaneous measurement of intracellular redox state and extracellular respiratory activity, yet integrated sensing approaches remain limited. Here, we present a dual fluorescent sensing platform combining a genetically encoded redox biosensor (roGFP2-Tsa2{Delta}CR) with an optical oxygen sensor embedded in microwell plates for parallel, noninvasive quantification of intracellular reactive oxygen species (ROS) and oxygen...
Integrated valorisation of PET and xylose using the oleaginous microorganisms Yarrowia lipolytica and Rhodococcus jostii
The increasing accumulation of polyethylene terephthalate (PET) waste has prompted the development of sustainable biotechnological strategies for its degradation and valorisation. This study presents an integrated approach combining enzymatic PET depolymerization by Yarrowia lipolytica, engineered to express and secrete the cutinase HiC and the lipase CalB, with the microbial valorization of PET-derived monomers, terephthalic acid (TPA) and ethylene glycol (EG), by Rhodococcus jostii RHA1....
Developing engineering strategies to enhance the genetic stability of fatty alcohol-producing strains for production scale-up
Robust strain stability is essential for industrial-scale fatty alcohol production, as metabolic burden and toxicity not only constrain productivity but also create selective pressure for low-producing or non-producing subpopulations. This genetic instability leads to genetic heterogeneity and compromises strain performance during large-scale fermentation. In this study, we investigated the production stability of fatty alcohol-producing Yarrowia lipolytica strains and developed systematic...