From flat moss to forests and flowers: Protein discovery may explain how plants conquered land

From flat moss to forests and flowers: Protein discovery may explain how plants conquered land Sadie Harley Scientific Editor Robert Egan Associate Editor If plants had never learned to grow in multiple directions, our world would look very different. No trees, flowers, or other complex plants—and therefore no animals or humans. New research from the University of Copenhagen now suggests that a specific protein in moss may have been crucial for this key step in plant evolution—a step that made life on land possible. The study is published in the journal New Phytologist. Around 470 million years ago, plant cells developed the ability to divide into three dimensions and grow upwards and sideways. Until then, as aquatic organisms, they had only grown in a two-dimensional flat form, limiting how complex they could evolve. The newly identified protein likely arose through evolution by combining two previously existing proteins into a single protein. The researchers do not yet know exactly when this fusion occurred, but it may have played a role in the early transition of plants to life on land. "We have identified a protein that has never been seen before and that has very special properties. It helps us gain a better understanding of how land plants function," explains one of the study's authors, Eleazar Rodriguez, Associate Professor in Functional Genomics at the Department of Biology. If you look at a tree today, its growth depends on fundamental biological mechanisms that emerged early in plant evolution. These include how cells divide in different directions, how cells obtain energy for growth, and how proteins are regulated within the cell. These are the mechanisms that the researchers now provide new insight into, dating back roughly 470 million years. "Without the ability to grow in three dimensions, the landscape would look very different. We would not see trees and shrubs grow the way they do today. Life on land would likely have remained much more limited," says Thomas Juel Ammitsøe, postdoc and co–first author of the study. Removing the newly discovered protein The researchers identified the previously unknown protein, named RAK1, in a moss species. The protein is a fusion between two types of proteins already known—a signaling protein (kinase) and an acetyltransferase. When present, it has a specific effect on the moss: by influencing the cell's energy metabolism, it enables cells to divide in multiple directions and form buds and shoots. This became clear when the researchers compared two versions of the same moss. In one, RAK1 was present; in the other, it had been removed. "We observed that cells in the moss lacking RAK1 did not divide properly and formed defective buds. This shows that RAK1 may have been crucial for enabling the moss to grow efficiently," explains the study's co–first author, assistant professor Cloe De Luxan Hernandez. Proteins are the workers of the cell Moss represents some of the earliest land plants that began to grow on Earth. Until now, the explanation for how moss developed the ability to grow in three dimensions has focused on gene regulation—specifically that certain genes are switched on and off at the right time. The researchers from the University of Copenhagen now build on this explanation by showing that simply turning genes on and off is not sufficient. The newly discovered RAK1 helps coordinate the metabolic balance needed for three-dimensional growth. The discovery of RAK1 highlights that evolution does not always invent something entirely new—sometimes it simply combines existing elements in new ways. "Our findings suggest that the transition from flat to three-dimensional plant growth depends not only on gene regulation, but also on precise metabolic control during stem cell division and bud formation," says Rodriguez. The discovery therefore provides not only new knowledge about moss, but also insight into fundamental mechanisms underlying growth in living organisms. Like human stem cells, moss stem cells depend on tightly controlled metabolism during growth and division. Our findings suggest that RAK1 is part of this regulatory system," concludes Rodriguez. Publication details Cloe de Luxán‐Hernández et al, An N‐acetyltransferase‐ MAPK fusion protein modulates developmental reprogramming in Physcomitrium patens, New Phytologist (2026). DOI: 10.1111/nph.71214 Journal information: New Phytologist Key concepts developmental biologymossesBiological EvolutionCellular organization, physiology & dynamicsProvided by University of Copenhagen